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Post-Transcriptional Gene Regulation
Edited by J Wilusz
Humana Press
2008
Hardcover 318 pp ISBN 9781588297839
£64.00
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In Post-Transcriptional Gene Regulation, renowned authors present current technical
approaches to most aspects of post-transcriptional control and provide a useful and versatile laboratory
bench resource. With chapters split into sections covering bioinformatics, fundamental aspects of the study
of RNA biology, and techniques for specific aspects of RNA biology, the expert authors have filled the book
with invaluable tricks of the trade, perfected in their state-of-the-art laboratories.
This new volume from the Methods in Molecular Biology series is convinently divided into three
sections. The first section presents a series of bioinformatic approaches to address the use of RNA
databases and algorithms to the study of post-transcriptional regulation involving untranslated regions
of transcripts. In the second section, a series of methods applicable to fundamental issues in mRNA
biology are presented. These include RNA structure/function, mRNP analysis and novel methods for
mRNA labeling and isolation. The third section of this volume presents methodologies to study particular
aspects of post-transcriptional control. This section includes methods for the study of alternative splicing
and 3€ end processing, mRNA localization, mRNA translation, mRNA stability and si/miRNA regulation.
Collectively, this book provides the reader with a useful and versatile laboratory bench resource
that will become an essential reference in the field.
Written for geneticists, cell biologists, bioinformaticians
Contents
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Bioinformatics Approaches for Studying Untranslated Regions of mRNAs
Paramjeet S. Bagga
- Identification of mRNA Polyadenylation Sites in genomes Using cDNA Sequences, Expressed Sequence Tags and Trace
Ju Youn Lee, Ji Yeon Park and Bin Tian
- Bioinformatic Tools for Studying Post-Transcriptional Gene Regulation: The UAlbany TUTR (Training UTR) Collection and other Informatic Resources
Francis Doyle, Christopher Zaleski, Alish D. George, Eric Stenson, Adele Ricciardi and Scott A. Tenenbaum
- In-Line Probing Analysis of Riboswitches
Elizabeth E. Regulski and Ronald R. Breaker
- Ribotrap: Targeted Purification of RNA-Specific RNPs from Cell Lysates via Immunoaffinity Precipitation to Identify Regulatory Proteins and RNAs
Dale L. Beach and Jack D. Keene
- Advances in RIP-Chip Analysis: RNA-Binding Protein Immunoprecipitation-
Microarray (Chip) Profiling
Timothy E. Baroni, Sridar V. Chittur, Ajish D. George and Scott A. Tenenbaum
- Biosensors for RNA Aptamer-Protein Interaction
Sara Tombelli, Maria Minunni and Marco Mascini
- A Tethering Approach to Study Proteins that Activate mRNA Turnover in Human Cells
Sandra L. Clement and Jens Lykke-Andersen
- RNA Analysis by Biosynthetic Tagging (RABT) Using 4-Thiouracil and Uracil Phosphoribosyltransferase
Gusti M. Zeiner, Michael D. Cleary, Ashley E. Fotus, Christopher D. Meiring, Edward S. Mocarski and John C. Boothroyd
- Efficient 5' Cap Dependent RNA Purification: Use in Identifying and Studying Subsets of RNA
Edyta Z. Bajak and Curt H. Hagedorn
- Enrichment of Alternatively Spliced Isoforms (EASI)
Julian P. Venables
- In Vivo Methods to Assess Polyadenylation Efficiency
Lisa K. Hague, Tyra Hall-Pogar and Carol S. Lutz
- Monitoring the Temporal and Spatial Distribution of RNA in Living Yeast Cells
Roy M. Long and Carl R. Urbinati
- Analysis of mRNA Partitioning Between the Cytosol and Endoplasmic Reticulum Compartments of Mammalian Cells
Samuel B. Stephens, Rebecca D. Dodd, Rachel S. Lerner, Brook M. Pyhtila and Christopher V. Nicchitta
- In Vivo and In Vitro Analysis of Poly(A) Length Effects on mRNA Translation
Jing Peng, Elizabeth L. Murray and Daniel R. Schoenberg...........................
- A Ribosomal Density Mapping (RDM) Procedure to Explore Ribosome Positions Along Translating mRNAs
Naama Eldad and Yoav Arava
- Identification of Changes in Gene Expression by Quantitation of mRNA Levels
Wendy M. Olivas
- Application of the InvaderR RNA Assay to the Polarity of Vertebrate mRNA Decay
Elizabeth L. Murray and Daniel R. Schoenberg
- Development of an In Vitro mRNA Decay System in Insect Cells
Kevin Sokoloski, John R. Andersen and Jeffrey Wilusz
- Using Synthetic Precursor and Inhibitor miRNAs to Understand miRNA Function
Lance P. Ford and Angie Cheng
- A Step-By-Step Procedure to Analyze the Efficacy of siRNA Using Real-Time PCR
Angie Cheng, Charles L. Johnson and Lance P. Ford
To find similar publications, click on a keyword below:
Humana Press
: biochemistry
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: cell biology
: genes
: transcription
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