Molecular Biomethods Handbook

Softcover  1124 pp  ISBN 9781603273749      £76.00

Part A of the book describes nucleic acid methods, such as gene expression profiling, microarray analysis and quantitative PCR.

In Part B, protein and cell-based methods are outlined, in subjects ranging from protein engineering to high throughput screening.

Written by a well-established panel of research scientists, Molecular Biomethods Handbook, 2nd Edition provides an up-to-date collection of methods used regularly in the authors€ own research programs. This book will prove to be an invaluable reference for those engaged in or entering the field of molecular biology, and will provide the necessary background for those interested in setting up and using the latest molecular techniques.

Of interest to molecular biologists, graduate students, undergraduate students, research scientists, technicians

Contents

PART A: NUCLEIC ACID BIOMETHODS

• The manipulation of nucleic acids: basic tools and techniques € Gayle Corkill and Ralph Rapley
• Restriction Enzymes € Gareth J.S. Jenkins
• Principles and Medical Applications of the Polymerase Chain Reaction € Birmal D.M. Theophilus
• Probe Design, Production, and Applications € Marilena Aquino de Muro
• Southern Blotting as a Diagnostic Method € Bronwen Harvey and Pirkko Soundy
• Introduction to Capillary Electrophoresis of DNA - Beatriz Sanchez-Vega
• Denaturing High-Performance Liquid Chromatography (DHPLC) for nucleic acid analysis - Kim Hung Leung and Shea Ping Yip
• Denaturing Gradient Gel Electrophoresis (DGGE) € Jeroen H. Roelfsema and Dorien J.M. Peters
• Single Strand Conformation Polymorphism (SSCP) Analysis - Kim Hung Leung and Shea Ping Yip
• Randomly amplified polymorphic DNA (RAPD); a useful tool for genomic characterization of different organisms - Lúcia Maria da Cunha Galvao and Eliane Lages-Silva
• Quantification of mRNA using Real Time RT-PCR € the SYBR solution - David Sugden and Patricia de Winter
• Quantitative Analysis of DNA Sequences by PCR and Solid Phase Sequencing € Anu Suomalainen and Ann-Christine Syvanen
• MAPH (Multiplex Amplifiable Probe Hybridization) - Carolina Sismani, Ludmila Kousoulidou and Philippos C. Patsalis
• Gene Expression Profiling - Arnis Druka, Robbie Waugh and Pete Hedley
• Comparative Genomic Hybridization in Clinical and Medical Research € Peng-Hui Wang, Yann-Jang Chen and Chi-Hung Lin
• Subtractive Hybridization € Craig Winstanley
• Fluorescence in situ Hybridization - Jane Bayani and Jeremy A. Squire
• Quantitative Trait Locus Mapping to Identify Genes for Complex Traits in Mice - Jonathan D. Smith
• cDNA Microarrays € Phillip G. Febbo
• Mapping Techniques € Simon G. Gregory
• Single Nucleotide Polymorphisms Overview € Nameeta Shah
• Bioinformatics Tools for Gene and Protein Sequence Analysis€ Bernd H.A. Rehm and Frank Reinecke

PART B: PROTEIN AND CELL METHODS

• Protein Electrophoresis - David Sheehan and Siobhan O€Sullivan
• Protein blotting - Patricia Gravel
• Capillary electrophoresis of proteins - Mark Strege
• Autoradiography and fluorography - Bronwen Harvey
• Mass spectrometry of proteins & peptides - Kenneth G. Standing
• Post-translational modifications - Christoph Kannicht and Birte Fuchs
• Protein microarray technology - Charlotte H. Clarke and Eric T. Fung
• Protein-protein interactions - Hae Ryoun Park, Lisa Montoya Cockrell, Yuhong Du, Andrea Kasinski, Jonathan Havel, Jing Zhao, Francisca Reyes-Turcu, Keith Wilkinson and Haian Fu
• Glycoprotein analysis - Terry Butters and David C.A. Neville
• Solid Phase Peptide Synthesis - Mare Cudic and Gregg B. Fields
• Monoclonal antibodies - Zhong J. Zhang and Maher Albitar
• Antibody Phage display - Rob Aitken
• Protein engineering - Thomas Willemsen, Urs B. Hagemann, Eva M. Jouaux, Sabine Stebel, Jody M. Mason, Kristian M. Müller and Katja M. Arndt
• Directed protein evolution - Sabine C. Stebel, Annette Gaida, Katja M. Arndt and Kristian M. Müller
• Enzyme Linked Immunosorbent Assay - John Crowther
• Epitope mapping - Glenn E. Morris
• Quantum dots - Charles Hotz
• Ion exchange chromatography - David Sheehan
• Size exclusion chromatography - Paul Cutler
• Hydrophobic interaction chromatography - Paul A. O€Farrell
• Affinity chromatography - Adam Charlton and Michael Zachariou
• High Performance Liquid Chromatography (HPLC) of Proteins and Peptides - Tzong-Hsien Lee and Marie-Isabel Aguilar
• Amino acid analysis - Ian Davidson + Paula O€Connor
• Surface plasmon resonance - K. Scott Phillips and Quan Cheng
• Macromolecular Crystallography - Bernhard Rupp and Kathrine A. Kantardjieff
• Microchip Devices for Bioanalysis - Anna Kinsella and Shelley D. Minteer
• Mammalian cell culture - Simon Langdon
• Plant tissue culture - V.M. Loyola Vargas, C. De-la-Peña, R.M. Galaz-Avalos and F. R. Quiroz-Figueroa
• Stem cells and Regenerative Medicine - Mohan C. Vemuri and Chellu S. Chetty
• Cryopreservation : Conservation of Bioresources at Ultra-Low Temperatures - J.G. Day, K. Harding, J. Nadarajan and E.E. Benson
• Magnetic resonance imaging (MRI) - Pottumarthi V. Prasad and Pippa Storey
• Electron microscopy - John Kuo
• Confocal microscopy - Guy Cox
• Laser microdissection - Graeme I. Murray
• Biomedical uses of Flow cytometry - James L. Weaver and Maryalice Stetler-Stevenson
• Immunomicroscopy - Constance Oliver
• In-situ hybridization - Ian A. Darby and Tim D. Hewitson
• Hi