Advanced Techniques in Soil Microbiology

Edited by Varma, Ajit; Oelmüller, Ralf 
Springer  2007  

Hardcover  427 pp  ISBN 9783540708643      £157.00
Advanced Techniques in Soil Microbiology presents a wide range of biotechnological methods for application in soil microbiology analysis. These include all essential methods involving molecular biology, immunology, microbiology, and structural biology, such as transcriptome analysis, RNAi technology, molecular matchmaking, RAPD, T-RFLP and FT/MS.

The techniques and procedures presented here have been selected with the aim of offering practical guides for immediate use in the laboratory. The systems investigated range from individual molecules and cells to entire eukaryotic organisms, with a focus on bacteria, fungi, mycorrhiza, and higher plants. This volume of state-of-the-art, practice oriented methods will be of great use both to the first-timer and to the experienced scientist.

Written for microbiologists, plant scientists, biotechnologists, soil scientists.


1 Detection and Diversity of Fungi from Environmental Samples: Traditional Versus Molecular Approaches, R. Jeewon and K.D. Hyde
2 Functional Genomic Approaches for Mycorrhizal Research, A. K. Pandey, H. White, and G.K. Podila
3 Automated Fluoroscence Sequencing and Troubleshooting, S. Gochhait, D. Malhotra, E. Rai, and R.N.K. Bamezai
4 mRNA Quantitation Using Real Time PCR, S. Gochhait, S.I. Bukhari, and R.N.K. Bamezai
5 Laboratory Practice for the Production of Polyclonal and Monoclonal Antibodies, S. Khurana, S. Bhaskar, and A. Mukhopadhyay
6 Modern Techniques for Analyzing Immunological Responses, Satish Khurana, Sangeeta Bhaskar, and Asok Mukhopdhyay
7 Transcriptome Analysis, S.K. Yadav, S.L. Singla-Pareek, and A. Pareek
8 RNAi Technology: a Tool for Functional Validation of Novel Genes, R. Karan, S. Kumari, S.K. Yadav, and A. Pareek
9 Molecular Matchmaking: Techniques for Biomolecular Interactions, R. Oberoi, P. Kumar, and S.K. Lal
10 Environmental Proteomics: Extraction and Identification of Protein in Soil, Z. Solaiman, M. A. Kashem and I. Matsumoto
11 DGGE and RISA Protocols for Microbial Community Analysis in Soil, Z. Solaiman and P. Marschner
12 Soil Microbial Community Structureand Function Assessed by FAME, PLFA and DGGE - Advantages and Limitations, P. Marschner
13 Measurement of Microbial Biomass and Activity in Soil, Z. Solaiman
14 Immuno-Technology for the Localization of Acid Phosphatase Using Native Gel Bands in Piriformospora indica and Other Soil Microorganisms, R. Malla, U. Pokharel, R. Prasad, R. Oelmüller, and A. Varma
15 Use of Short Oligonucleotide Primers in Random Amplified Polymorphic DNA Techniques for Species Identification, R. Malla and A. Varma
16 Co-Cultivation with Sebacinales, A.C. Kharkwal, R. Prasad, H. Kharkwal, A. Das, K. Bhatnagar, I. Sherameti, R. Oelmüller, and A. Varma
17 Quantitative Histochemistry: a Forgotten Tool with New Applications , R. Hampp and S. Haag
18 Ion Cyclotron Resonance Fourier Transform Mass Spectrometry for Non-Targeted Metabolomics of Molecular Interactions in the Rhizosphere, P. Schmitt-Kopplin, N. Hertkorn, M. Frommberger, M. Lucio, M. Englmann, A. Fekete, and I. Gebefugi
19 Application of Terminal-Restriction Fragment Length Polymorphism for MolecularAnalysis of Soil Bacterial Communities , A. Mengoni, E. Giuntini, and M. Bazzicalupo
20 Molecular Symbiotic Analysis Between Arabiopsis thaliana and Piriformospora indica , B. Shahollari, K. Bhatnagar, I. Sherameti, A. Varma, and R. Oelmüller
21 Biophysical Phenomics Reveals Functional Building Blocks of Plants Systems Biology: a Case Study for the Evaluation of the Impact of Mycorrhization with Piriformospora indica , R.J. Strasser, M. Tsimilli-Michael, D. Dangre, and M. Rai
22 Analysis of the Plant Protective Potential of the Root Endophytic Fungus Piriformospora indica in Cereals, F. Waller, B. Achatz, and K.-H. Kogel
23 Members of Sebacinales Confer Resistance Against Heavy Metal Stress in Plants , N. Hahn, A. Varma, R. Oelmüller, and I. Sherameti
24 Screening of Plant Growth-Promoting Rhizobacteria, C.S. Nautiyal and S.M. DasGupta
25 Research Methods in Arbuscular Mycorrhizal Fungi, A. Gaur and A. Varma
26 Field Trials of Bioinoculants, I. Orta? and A. Varma
Subject Index .

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Rhizobium : Springer : biology, general : environmental science : genomics : immunology : microbiology : mycology : mycorrhizae : proteomics : soil science

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