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Baculovirus and Insect Cell Expression Protocols - 2nd edition
Edited by Murhammer, David W.
Humana Press
2007
Hardcover 470 pp ISBN 9781588295378
£106.00
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This second edition is a detailed guide for using the baculovirus expression vector system (BEVS)
and/or insect cells to produce recombinant proteins. Procedures for the development and production of
baculovirus insecticides at both laboratory and large scale are described in the kind of step-by-step format
that the Methods in Molecular Biology series helped standardize.
This book is a one-stop source for information on baculoviruses, and includes sections on using modified
baculoviruses to express genes in mammalian cells, using Drosophila cell lines, isolating new cell lines, and
developing serum-free medium. Specific applications, including using baculovirus and insect cell system
to study apoptosis, are also discussed.
This updated and expanded edition provides a perfect lab manual for biochemists, molecular biologists,
biochemical engineers, and others for using the BEVS or insect cell lines to produce recombinant proteins.
Of interest to: Biochemists, molecular and cell biologists, biochemical engineers
Contents
PART I. INTRODUCTION
Chapter 1 Useful Tips, Widely Used Techniques and Quantifying Cell Metabolic Behavior -
David W. Murhammer
PART II. BACULOVIRUS MOLECULAR BIOLOGY/DEVELOPMENT OF RECOMBINANT BACULOVIRUSES
Chapter 2 Introduction to Baculovirus Molecular Biology -
Barbara J. Kelly, Linda A. King, and Robert D. Possee
Chapter 3 Baculovirus Transfer Vectors -
Robert D. Possee and Linda A. King
Chapter 4 Recombinant Baculovirus Isolation -
Linda A. King, Richard Hitchman, and Robert D. Possee
Chapter 5 Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System -
James A. Fornwald, Quinn Lu, Dayuan Wang, and Robert S. Ames
PART III. INSECT CELL CULTURE
Chapter 6 Available Lepidopteran Insect Cell Lines -
Dwight E. Lynn
Chapter 7 Lepidopteran Insect Cell Line Isolation From Insect Tissue -
Dwight E. Lynn
Chapter 8 Development of Serum-Free Media for Lepidopteran Insect Cell Lines -
Sprios N. Agathos
Chapter 9 Routine Maintenance and Storage of Lepidopteran Insect Cell Lines and Baculoviruses -
Dwight E. Lynn
PART IV. PROTEIN PRODUCTION WITH RECOMBINANT BACULOVIRUSES
Chapter 10 Small Scale Protein Production With the Baculovirus Expression Vector System -
Steve Harwood
Chapter 11 Recombinant Protein Production in Large Scale Agitated Bioreactors Using the Baculovirus
Expression Vector System -
Cynthia B. Elias, Barbara Jardin, and Amine Kamen
Chapter 12 Production of Baculovirus-Expressed Recombinant Proteins in Wave Bioreactors -
Sue H. Kadwell and Philip I. Hardwicke
Chapter 13 Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae -
Yi Liu, Nathan DeCarolis, and Nikolai van Beek
Chapter 14 Virus-Like Particle and Viral Vector Production Using the BEVS/Insect Cell System: Adeno-Associated
Virus Based Products -
Marc G. Aucoin, Danielle Jacob, Parminder S. Chahal, Jamal Meghrous, Alice Bernier, and Amine A. Kamen
PART V. RECOMBINANT PROTEIN PRODUCTION WITH TRANSFORMED INSECT CELLS
Chapter 15 Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression -
Robert L. Harrison and Donald L Jarvis
Chapter 16 Transformation of Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression -
Lucy Cherbas and Peter Cherbas
Chapter 17 Transforming Lepidopteran Insect Cells for Improved Protein Processing -
Robert L. Harrison and Donald L Jarvis
PART VI. BACULOVIRUS DEVELOPMENT AND PRODUCTION FOR USE AS INSECTICIDES
Chapter 18 Introduction to the Use of Baculoviruses as Biological Insecticides -
Bryony C. Bonning and Tyasning Nusawardani
Chapter 19 Baculovirus Insecticide Production in Insect Larvae -
Nikolai van Beek and David C. Davis
Chapter 20 Evaluation of the Insecticidal Efficacy of Wild Type and Recombinant Baculoviruses -
Huarong Li and Bryony C. Bonning
PART VII. MISCELLANEOUS TECHNIQUES AND APPLICATIONS OF THE BACULOVIRUS/INSECT CELL SYSTEM
Chapter 21 Monitoring and Visualization of Baculovirus Infection Using Green Fluorescent Protein Strategy -
Hyung Joon Cha and William E. Bentley
Chapter 22 Alternative Molecular and Reactor Strategies Probing Infection and Production -
Yu-Chen Hu and William E. Bentley
Chapter 23 Methods For Gene Silencing with RNAi -
John C. March and William E. Bentley
Chapter 24 Use of the Baculovirus/Insect Cell System to Study Apoptosis -
Nor Chejanovsky
Chapter 25 Generation of Envelope-Modified Baculoviruses for Gene Delivery Into Mammalian Cells -
Christian Hofmann
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