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Nucleic Acids and Proteins in Soil
Edited by Nannipieri, Paolo; Smalla, Kornelia
Springer
2006
Hardback 458 p., 42 illus. ISBN 3540294481
£115.00
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With millions of different bacterial species living in soil, the microbial community is extremely complex, varying
at very small scales. Microbe-driven functions are essential for most processes in soil. Thus, a better understanding
of this microbial diversity will be invaluable for the management of the various soil functions.
Nucleic Acids and Proteins in Soil combines traditional approaches in soil microbiology and biochemistry with the
latest techniques in molecular microbial ecology. Included are methods to analyse the presence and importance
of nucleic acids and proteins both inside and outside microbial cells, the horizontal gene transfer which drives
bacterial diversity, as well as soil proteomes. Further chapters describe techniques such as PCR, fingerprinting,
the challenging use of gene arrays for structural and functional analysis, stable isotope probing to identify in
situ metabolic
functions, and the use of marker and reporter genes in soil microbial ecology.
Of interest to microbiologists, agriculturalists, soil biologists, biochemists
Contents
1 New Frontiers in SoilMicrobiology:
How To Link Structure and Function of Microbial Communities?
Bla-Stres, James M. Tiedje
2 Chemical Structure of Organic N and Organic P in Soil
Ingrid Kögel-Knabner
3 Nucleic Acid Extraction fromSoil
Lars R. Bakken, Åsa Frostegård
4 Role of Stabilised Enzymes in Microbial Ecology
and Enzyme Extraction fromSoil with Potential Applications
in Soil Proteomics Paolo Nannipieri
5 Soil Proteomics: Extraction and Analysis of Proteins from Soils
Oladele A. Ogunseitan
6 The Various Sources and the Fate of Nucleic Acids in Soil
Wilfried Wackernagel
7 Stabilization of Extracellular DNA and Proteins
by Transient Binding to Various Soil Components
Kaare M. Nielsen,Luca Calamai,Giacomo Pietramellara
8 Assessing Bacterial and Fungal Community Structure in Soil
Using Ribosomal RNA and Other Structural GeneMarkers
George A. Kowalchuk,Barbara Drigo,Etienne Yergeau,
Johannes A. van Veen
9 Advances in Microarray-Based Technologies
for SoilMicrobial Community Analyses
ChristopherW. Schadt, Jizhong Zhou
10 Stable Isotope Probing: A Critique of Its Role in Linking Phylogeny and Function
Mike Manefield,Robert I. Griffiths,Mark J. Bailey,Andrew S. Whiteley
11 Gene Detection, Expression and Related Enzyme Activity in Soil
Martin Krsek,William H. Gaze, N.Z. Morris,Elizabeth M.H.Wellington
12 Enzyme Activities in Soil
Liliana Gianfreda, Pacifico Ruggiero
13 How to Assess the Abundance and Diversity
of Mobile Genetic Elements (MGE)
in Soil Bacterial Communities?
Kornelia Smalla, Holger Heuer
14 Bacterial Conjugation in Soil
Jan Dirk van Elsas, Sarah Turner, Jack T. Trevors
15 Horizontal Gene Transfer by Natural Transformation
in Soil Environment
Anne Mercier, Elisabeth Kay, Pascal Simonet
16 Reporter Genes in Bacterial Inoculants
Can Monitor Life Conditions and Functions in Soil
Jan Sorensen, Ole Nybroe
17 Reporter Gene Technology in Soil Ecology;
Detection of Bioavailability and Microbial Interactions
Mette Burmolle,Lars Hestbjerg Hansen, Soren J. Sorensen
18 Marker Genes As Tools To Study
Deliberately Released Soil Bacteria
Christoph C. Tebbe, Rona Miethling-Graff
Subject Index
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