Microbial Proteomics: Functional Biology of Whole Organisms

Hardcover  512 pages  ISBN 0471699756      £105.00

This text provides an exhaustive analysis and presentation of current research in the field of microbial proteomics, with an emphasis on new developments and applications and future directions in research. The editors and authors show how and why the relative simplicity of microbes has made them attractive targets for extensive experimental manipulation in a quest for both improved disease prevention and treatment and an improved understanding of whole organism functional biology. In particular, the text demonstrates how microbial proteomic analyses can aid in drug discovery, including identification of new targets, novel diagnostic markers, and lead optimization.

Each chapter is written by one or more leading experts in the field and carefully edited to ensure a consistent and thorough approach throughout. Methods, technologies, and tools associated with the most promising approaches are stressed. Key topics covered include:

• Microbial pathogenesis at the proteome level
• Whole cell modeling
• Structural proteomics and computational analysis
• Biomolecular interactions
• Physiological proteomics
• Metabolic reconstruction using proteomics data

While presenting the practical utility of proteomics data, the text is also clear on the field's current limitations, pointing to areas where further investigation is needed.

Offering a state-of-the-art perspective from internationally recognized experts, this text is ideally suited for researchers and students across the gamut of genomic sciences, including biochemistry, microbiology, molecular biology, genetics, biomedical and pharmaceutical sciences, biotechnology, and veterinary science.

Contents

PART I: EDITORAL / PREFACE.

PART II: GENERAL PROTEOMICS OF MICROORGANISMS/MODEL ORGANISMS.

• 1. Holistic Biology of Microorganisms: Genomics, Transcriptomics and Proteomics (V. Wasinger). 2. Strategies for Measuring Dynamics: The Temporal Component of Proteomics (R. Benyon & J. Pratt). 3. The Quest for Complete Proteome Coverage (D. O'Connor, et al.). 4. The Proteome of Mycoplasma Pneumoniae (R. Herrmann & T. Ruppert). 5. Proteomics of Archaea (R. Cavicchioli, et al.).

PART III: PROTEOMICS AND CELL PHYSIOLOGY.

BASIC PHYSIOLOGY, STRESS AND STARVATION RESPONSES.

• 6. Elucidation of the Mechanisms of Acid Stress in Listeria Monoctogenes by Proteomic Analysis (L. Phan-Thanh & L. Jänsch). 7. Oxidation of the Bacterial Proteome in Response to Starvation (T. Nyström). 8. A Tale of Two Metal Reducers: Comparative Proteome Analysis of Geobacter Sulferreducens PCA and Shewanella Oneidensis MR-1 (C. Giometti). 9. The AMT Tag Approach to Proteomic Characterization of Deinococcus Radiodurans and Shewanella Oneidensis (M. Lipton, et al.).

PHYSIOLOGICAL PROTEOMICS OF INDUSTRIAL BACTERIA.

• 10. The Proteomics Corynebacterium Glutamicum-An Essential Industrial Bacterium (A. Burkovski). 11. Proteomics of Lactococcus Lactis: Phenotypes for a Domestic Bacterium (M. Kilstrup). 12. A Proteomic Survey Through the Secretome of Bacillus Subtilis (H. Antelmann).

PART IV: PROTEOMICS OF PATHOGENIC MICROORGANISMS.

BACTERIAL PATHOGENESIS.

• 13. Analysing Bacterial Pathogenesis at the Level of the Proteome (P. Cash). 14. Unraveling Edwardsiella Tarda Pathogenesis Using the Proteomics Approach (P. Rao, et al.). 15. Structural Proteomics and Computational Analysis of a Deadly Pathogen: Combating M. Tuberculosis from Multiple Fronts (M. Strong & C. Gouldiing).

EUKARYOTIC PATHOGENS.

• 16. Proteomic Studies of Plant-Pathogenic Oomycetes and Fungi (C. Bruce, et al.). 17. candida Albicans Biology and Pathogenicity: Insights from Proteomics (A. Pitarch, et al.). 18. Contributions of Proteomics to Diagnosis, Treatment and Prevention of Candidiasis (A. Pitarch, et al.).

VACCINE DEVELOPMENT.

• 19. Identification of Protein Candidates for Developing Bacterial Ghost Vaccines Against Brucella (A. Khan, et al.). 20. Genomics and Proteomics in Reverse Vaccines (G. Grandi).

PART V: PROTEOME DATABASES, BIOINFORMATICS AND BIOCHEMICAL MODELLING.

• 21. Databases and Resources for In Silico Proteome Analysis (M. Pruess, et al.). 22. Inter and Intraspecies Comparison of Microbial Proteins: Learning About Gene Ancestry Function and Species Life Style (B. Labedan & O. Lespinet). 21. Cellular Modelling of Biochemical Processes in Microorganisms (I. Goryanin, et al.).