Phage Display - A Practical Approach

Paperback  256 pages  ISBN 019963873X      £65.00

• "Modular" chapters on making libraries, performing selections, and analyzing clones
• Extensively cross-referenced
• Draws on work from a variety of leading labs in the field

Readership: 1) Graduate students and postdocs embarking on a phage display project 2) Principal Investigators who are considering phage display projects or who are relatively new to the field-could be basic researchers, clinical researchers, or researchers in drug discovery (biotech/pharma) 3) Established phage display users 4) Curious chemists

Contents

• Introduction to phage biology and phage display , Russel, Lowman and Clackson
• Constructing phage display libraries by oligonucleotide-directed mutagenesis , Sidhu and Weiss
• In vitro DNA recombination , Miyazaki and Arnold
• Phage selection strategies for improved affinity and specificity of proteins and peptides , Dennis and Lowman
• Rapid screening of phage displayed protein binding affinities by phage ELISA , DeLano and Cunningham
• Identification of novel ligands for receptors using recombinant peptide libraries , Cwirla et al
• Substrate phage display , Matthews and Ballinger
• Protease-based selection of stably folded proteins and protein domains from phage display libraries , Tuna et al
• Phage display of zinc fingers and other nucleic acid-binding motifs , Isalan and Choo
• In vivo and ex vivo selections using phage-displayed libraries , Hoffman et al
• Screening phage libraries with sera , Monaci and Cortese
• Interaction cloning using cDNA libraries displayed on phage , Jespers and Fransen
• Phage antibody libraries , Bradbury and Marks
• Affinity maturation of phage antibodies , Neilson and Marks

To find similar publications, click on a keyword below:
DNA : Oxford University Press : antibodies : biochemistry : biotechnology : phage : proteins