Principles of Gene Manipulation and Genomics (7th edition)

Paperback  668 pages, 427 illustrations.  ISBN 1405135441      £30.00

• Comprehensively revised, updated and rewritten to encompass within one volume, basic and advanced gene manipulation techniques, genome analysis, genomics, transcriptomics, proteomics and metabolomics
• Includes two new chapters on the applications of genomics
• An accompanying website - www.blackwellpublishing.com/primrose - provides instructional materials for both student and lecturer use, including multiple choice questions, related websites, and all the artwork in a downloadable format.
• An essential reference for upper level undergraduate and graduate students of genetics, genomics, molecular biology and recombinant DNA technology.

Contents

Preface

• 1. Gene Manipulation in the Post-Genomics Era

Part I: Fundamental Techniques of Gene Manipulation:

• 2. Basic Techniques
• 3. Cutting and Joining DNA Molecules
• 4. Basic Biology of Plasmid and Phage Vectors
• 5. Cosmids, Phasmids and Other Advanced Vectors
• 6. Gene Cloning Strategies
• 7. Sequencing Genes and Short Stretches of DNA
• 8. Changing Genes: Site-directed Mutagenesis and Protein Engineering
• 9. Bioinformatics

Part II: Manipulating DNA in Microbes, Plants and Animals:

• 10. Cloning in Bacteria Other Than Escherichia coli
• 11. Cloning in Saccharomyces cerevisiaea and Other Fungi
• 12. Gene Transfer to Animal Cells
• 13. Genetic Manipulation of Animals
• 14. Gene Transfer to Plants
• 15. Advanced Transgenic Technology

Part III: Genome Analysis, Genomics and Beyond:

• 16. The Organization and Structure of Genomes
• 17. Mapping and Sequencing Genomes
• 18. Comparative Genomics
• 19. Large-Scale Mutagenesis and Interference
• 20. Analysis of the Transcriptome
• 21. Proteomics I - Expression Analysis and Characterization of Proteins
• 22. Proteomics II - Analysis of Protein Structures
• 23. Proteomics III - Protein Interactions
• 24. Metabolomics and Global Biochemical Networks

Part IV: Applications of Gene Manipulation and Genomics:

• 25. Applications of Genomics: Understanding the Basis of Polygenic Disorders and Identifying Quantitative Trait Loci
• 26. Applications of Recombinant DNA Technology