RNAi: A Guide to Gene Silencing

Edited by Gregory J. Hannon
CSHL 2003

Hardback 436 pp ISBN 0879696419 £75.00

Paperback 436 pp ISBN 0879697040 £45.00

The ability of double stranded RNA to inhibit the expression of genes with an appropriate sequence can be harnessed to silence target genes in vitro in a new and powerful way. This volume combines reliable RNAi protocols for a variety of species with discussion of strategies for the effective design of experiments using this important new technique that is changing the way experimental science is done.

Contents

Chapter 1. Sense Cosuppression in Plants: Past, Present, and Future Richard A. Jorgensen
Chapter 2. Transgene Cosuppression in Animals James A. Birchler, Manika Pal-Bhadra, and Utpal Bhadra
Chapter 3. Regulation of the Genome by Double€stranded RNA Marjori Matzke, M. Florian Mette, Tatsuo Kanno, Werner Aufsatz, and Antonius J.M. Matzke
Chapter 4. RNAi in Caenorhabditis elegans René F. Ketting, Marcel Tijsterman, and Ronald H.A. Plasterk
Chapter 5. Small RNAs, Big Biology: Biochemical Studies of RNA Interference Gregory J. Hannon and Phillip D. Zamore
Chapter 6.shRNA-mediated Silencing of Mammalian Gene Expression Michael T. McManus and Douglas S. Conklin
Chapter 7. Transposable Elements, RNA Interference, and the Origin of Heterochromatin Rob Martienssen, Tom Volpe, Zach Lippman, Ann-Valerie Gendrel, Catherine Kidner, Pablo Rabinowicz, and Vincent Colot
Chapter 8. The Ribonuclease III Superfamily: Forms and Functions in RNA Maturation, Decay, and Gene Silencing Allen W. Nicholson
Chapter 9. RNA-dependent RNA Polymerase in Gene Silencing Luyun Huang, John Gledhill, and Craig E. Cameron
Chapter 10. Structure and Function of Heterochromatin: Implications for Epigenetic Gene Silencing and Genome Organization Ira M. Hall and Shiv I.S. Grewal
Chapter 11. Total Interference: Genome-wide RNAi Screens in C. elegans Andrew G. Fraser and Julie Ahringer
Chapter 12. PTGS Approaches to Large Scale Functional Genomics in Plants Tessa M. Burch€Smith, Jennifer L. Miller, and S.P. Dinesh-Kumar
Chapter 13. Mammalian RNA Interference Thomas Tuschl
Chapter 14. RNAi in Avian Embryos Esther T. Stoeckli
Chapter 15. Guide to RNAi in Mouse Oocytes and Preimplantation Embryos Paula Stein and Petr Svoboda
Chapter 16. RNAi Technologies in Drosophila Cell Culture Scott M. Hammond
Chapter 17. RNAi Applications in Drosophila melanogaster Richard W. Carthew
Chapter 18. RNA Interference in Trypanosoma brucei and Other Nonclassical Model Organisms Elisabetta Ullu and Christian Tschudi

To find similar publications, click on a keyword below:
RNA : animal science : genes : methods : molecular biology : nucleic acids : plant science

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