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Proteins and Proteomics: A Laboratory Manual
Richard Simpson
CSHL
2003
Paperback 926 pp ISBN 0879695544
£100.00
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Completion of sequencing of the human genome, as well as those of many other organisms, has now opened the door
for exploration of the proteome, the many thousands of interacting proteins in a given organism. Critical to
tackling the complexity of the proteome is a workable strategy using reliable and tested protocols for identifying,
isolating, and quantifying proteins in cells and cell pathways and for performing functional assays.
Proteins and
Proteomics: A Laboratory Manual, authored by Richard Simpson of the Ludwig Institute for Cancer Research,
Melbourne, provides the first authoritative and wide€ranging protocol-based approach to proteomics.
Presented as a logical strategy for analyzing proteomes, Proteins and Proteomics provides information about protein
structure and numerous methods for the preparation and analysis of protein samples ranging from electrophoresis and
mass spectrometry to protein chips and informatics. Extensive background information and references are
provided regarding the theoretic aspects of the techniques presented as well as their applications.
This manual is an essential laboratory tool for genetics and molecular biology investigators moving from studies
of genomics and genotype to those of proteomics and phenotype. It is invaluable for cell biologists,
developmental biologists, and neuroscientists exploring the intricacies of the proteome. It will also be an
excellent resource for protein chemists and biochemists.
The website site, www.proteinsandproteomics.org, is a companion to the book and provides
free access to downloadable versions of all the figures found in the book and references to work
discussed in the footnotes as well as additional protocols and links to other on€line resources.
Contents
- Chapter 1. Introduction to Proteomics
- Chapter 2. One€dimensional Polyacrylamide Gel Electrophoresis
- Chapter 3. Preparing Cellular and Subcellular Extracts
- Chapter 4. Preparative Two€dimensional Gel Electrophoresis with Immobilized pH Gradients
- Chapter 5. Reversed€phase High€performance Liquid Chromatography
- Chapter 6. Amino€ and Carboxy€ terminal Sequence Analysis
- Chapter 7. Peptide Mapping and Sequence Analysis of Gel€resolved Proteins
- Chapter 8. The Use of Mass Spectrometry in Proteomics
- Chapter 9. Proteomic Methods for Phosphorylation Site Mapping
- Chapter 10. Characterization of Protein Complexes
- Chapter 11. Making Sense of Proteomics: Using Bioinformatics to Discover a Protein?s Structure, Functions,
and Interactions
To find similar publications, click on a keyword below:
Cold Spring Harbor
: biochemistry
: cell biology
: handbooks
: proteins
: proteomics
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