Purifying Proteins for Proteomics: A Laboratory Manual

Edited by Richard J. Simpson
CSHL 2004

Paperback 800 pp ISBN 0879696966 £90.00

Hardback 800 pp ISBN 0879696958 £130.00

This manual complements Simpson€s Proteins and Proteomics manual, with a comprehensive collection of methods for protein purification from a variety of source preparations. The chapters include detailed protocols, methods for optimizing the performance of experiments, discussion of potential pitfalls, and troubleshooting advice.

Contents

SECTION I Preparing Proteins for Proteomic Analysis

Chapter 1: Role of Separation Science in Proteomics Richard J. Simpson
Chapter 2: Strategies for Purifying Proteins Richard J. Simpson

SECTION II Chromatographic Methods for Preparing Proteins for Proteomics

Chapter 3: Introduction to Chromatographic Methods for Protein and Peptide Purification Richard J. Simpson
Chapter 4: Multidimensional Chromatography of Intact Proteins Alex Apffel
Chapter 5: High-throughput Screening of Soluble Recombinant Proteins Ting-Fang Wang and Andrew H.-J. Wang
Chapter 6: Ion-exchange Chromatography Bengt Westerlund
Chapter 7: Size-exclusion Chromatography Helena Hedlund
Chapter 8: Role of Reversed-phase High-performance Liquid Chromatography in Protein Isolation and Purification C. David Carr and Robert L. Moritz
Chapter 9: Hydrophobic Interaction Chromatography Richard J. Simpson and Paul A. O'Farrell
Chapter 10: Affinity and Immunoaffinity Chromatography Keith Brocklehurst, Albert J. Courey, Sheraz Gul, Sue Hwa Lin, and Robert L. Moritz
Chapter 11: Dye€Ligand Affinity Chromatography in Proteomic Research Nikos E. Labrou
Chapter 12: Immobilized Metal-ion Affinity Chromatography Lars Haneskog
Chapter 13: Protein Chromatography on Hydroxyapatite Richard J. Simpson
Chapter 14: Chromatofocusing Jamil Mohammad

SECTION III Electrophoretic Methods for Preparing Proteins for Proteomics

Chapter 15: Introduction to Strategies Based on Electrophoresis for Separating Proteins Richard J. Simpson
Chapter 16: Separation of Proteins Using Two-dimensional Gel Electrophoresis Angelika Görg, Oliver Drews, and Walter Weiss
Chapter 17: Sample Preparation for High-resolution Two-dimensional Electrophoresis by Isoelectric Fractionation in an MCE Ben R. Herbert, Pier Giorgio Righetti, John McCarthy, Jasmine Grinyer, Annalisa Castagna, Matthew Laver, Matthew Durack, Gerard Rummery, Robecca Harcourt, and Keith L. Williams
Chapter 18: An Electrophoresis Method for Purifying Proteins: The Gradiflow BF400 Instrument Vicki L. Locke and Theresa M. Thomas
Chapter 19: Protein Purification Using Free-flow Electrophoresis Peter J.A. Weber, Gerhard Weber, and Christoph Eckerskorn

SECTION IV Analysis of Purified Proteins Prior to Structural Proteomics

Chapter 20: Introduction to Methods for Characterizing Purified Proteins Richard J. Simpson
Chapter 21: Characterization of Proteins by Mass Spectrometry Gavin E. Reid
Chapter 22: Analytical Ultracentrifugation: Protein Size, Conformation, and Interactions Christopher A. MacRaild and Geoffrey J. Howlett
Chapter 23: Measuring the Conformational Stability of a Protein Gerald R. Grimsley, Beatrice M.P. Huyghues€Despointes, C. Nick Pace and J. Martin Scholtz
Chapter 24: Combining Surface Plasmon Resonance with Mass Spectrometry: Identifying Binding Partners and Characterizing Interactions Jos Buijs and Tohru Natsume
Chapter 25: Analysis of Carbohydrate from Glycoproteins David Oxley, Graeme Currie, and Antony Bacic

APPENDICES

A1: An Overview of Protein Structure Richard J. Simpson
A2: Measuring the Concentration of Proteins Richard J. Simpson
A3: Concentrating Solutions of Protein Richard J. Simpson
A4: Stabilizing Proteins for Storage Richard J. Simpson
A5: One-dimensional Polyacrylamide Gel Electrophoresis
A6: Troubleshooting Guides HPLC 2D Gels Cautions
Index

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Cold Spring Harbor : biochemistry : cell biology : handbooks : proteins : proteomics

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