|
Protein Folding Kinetics - Biophysical Methods (Second edition revised and extended)
B Nölting
Springer
2005
Hardcover 222 pp ISBN 3540272771
£63.00
|
|
|
|
|
|
Protein Folding Kinetics - Biophysical Methods (2nd Edition) gives a deep insight into the principles and concepts
of the kinetic and structural resolution of fast chemical and biophysical reactions of proteins with emphasis on
protein-folding reactions. The study of fast protein-folding reactions and the understanding of the folding paradox
have significantly advanced due to the recent development of new biophysical methods which allow not only kinetic
resolution in the sub-millisecond time scale but also structural resolution with unprecedented precision. Pathways
and structures of early and late folding events and the transition state structures of fast- and ultrafast-folding proteins
can now be studied in far more detail. Important techniques include biophysical, chemical, molecular biological and
mathematical methods, in particular protein engineering, Phi-value analysis, time-resolved circular dichroism, optical
triggers and pulsed infrared LASER methods, pressure and temperature jump, ultrafast mixing, stopped flow and
quenched flow, dielectric relaxation and electric-field-jump, acoustic relaxation, fluorescence- and isotope-labeling,
H/D exchange methods, NMR line broadening and stopped-flow NMR, transition state theory, solutions of rate
equations, and evolutionary computer programming. Protein Folding Kinetics - Biophysical Methods is written for
students and researchers in biochemistry, biophysics, and related fields.
Special features in the second edition:
-
- Includes detailed information and 12 color figures on the high resolution of folding transition states.
- Discusses structural determinants of the rate of protein folding on a timescale from microseconds to seconds.
- Provides information on self-evolving computer programs for protein-folding simulations and protein-structure
predictions.
Written for practitioners, scientists, lecturers, graduate students in biochemistry, biotechnology, biophysics
Contents
1 Introduction
2 Structures of proteins
3 Physical interactions that determine
the properties of proteins
4 Calculation of the kinetic rate constants
5 High kinetic resolution of protein folding events
6 Kinetic methods for slow reactions
7 Resolution of protein structures in solution
8 High structural resolution of transient
protein conformations
9 Experimental problems of the kinetic and structural
resolution of reactions that involve proteins
10 The folding pathway of a protein (barstar) at the
resolution of individual residues
from microseconds to seconds
11 Highly resolved folding pathways and mechanisms
of six proteins
12 Structural determinants of the rate of protein folding
13 Evolutionary computer programming of protein structure
and folding
Index
To find similar publications, click on a keyword below:
Springer
: biochemistry
: biophysics
: kinetics
: methods
: proteins
|
