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HPLC: Practical and Industrial Applications, Second Edition
Edited by Joel K Swadesh
CRC Press
2000
Hardback 480 ISBN 0849300037
£68.00
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- Presents highly conceptual information in plain language, making it accessible to students and easy to read for
experienced scientists
- Includes a comprehensive review of HPLC literature, eliminating the need for multiple references
- Provides extensive indexing and a careful structure to facilitate rapid answers to urgent problems
- Integrates important non-technical issues with the realities of the workplace, including discussion of a range
of topics, from management theory to regulatory considerations and ethicsNew in the Second Edition:
- Updates of all major topics highlighting recent literature and new developments
- Expanded discussion of mass spectroscopy, electrophoretic separations, and ultra-micro separations
- Further discussion on the nature and future of industrial development
Product specifications, regulatory constraints, and tight production schedules impose considerable pressures on
separation scientists in industry. The first edition of HPLC: Practical and Industrial Applications helped eliminate
the need for extensive library or laboratory research when confronting a problem, an unfamiliar technique,
or work in a new area. Its plain language, comprehensive coverage of separation topics, and practical
organization made it an accessible and convenient reference manual for anyone working in or just entering the field.
Since its publication in 1997, however, much has changed. The areas of mass spectroscopy, electrophoretic
separations, and ultra-micro separations have blossomed, focus on quality control has intensified, and the
literature has grown significantly. The Second Edition incorporates all of these changes and more. It is now
fully current, with chapter supplements that include updated references and discussions of techniques.
This book examines analytical HPLC as it is actually used in industry. Whether you are just entering industry,
switching from one industry to another, or simply enjoy understanding how things are made, HPLC: Practica
and Industrial Applications will help you solve problems and get up to speed in new areas quickly, comfortably,
and with a genuine sense of mastery.
Contents
INTRODUCTION, Jeffrey R. Larson, James E. Tingstad, and Joel K. Swadesh
Overview
Pumps
Columns
Chromatographic modes
Detectors
Chromatographic theory
Laboratory operations
The role of laboratory management
AUTOMATED SAMPLING IN THE PROCESS ENVIRONMENT, Jeffrey R. Larson
Issues in process HPLC
Case histories
Future directions for on-line HPLC process monitors
Summary
CHROMATOGRAPHY IN PROCESS DEVELOPMENT, Patricia Puma
Scope of process chromatography
Special hardware requirements
Validation considerations
Case history
Summary
REVERSED PHASE HPLC, Rekha D. Shah and Cynthia A. Maryanoff
Introduction
Chromatographic practice in RPLC
Selected applications
Case history: optimizing a chemical synthesis by RPLC
Conclusions
ION EXCHANGE CHROMATOGRAPHY, Joel K. Swadesh
Introduction
Ion exchange stationary phases
Electrochemical detectors
Applications
Case history: isolation and partial characterization of hementin, a compound of biopharmaceutical interest
Acknowledgments
GEL PERMEATION CHROMATOGRAPHY, Rajesh G. Beri, Laurel S. Hacche, and Carl F. Martin
Introduction
Detectors for gel permeation chromatography
Application of aqueous size-exclusion chromatography352 to carbohydrates: a case history
HIGH PERFORMANCE CAPILLARY ELECTROPHORESIS:
AN OVERVIEW, Kálmán Benedik and András Guttman
Introduction
Fundamentals
Instrument
Separation modes
Applications
Conclusions
Index
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