Handbook of Affinity Chromatography, Second Edition

Hardback  968pp  ISBN 0824740572      £110.00

• Presents the latest research in the theory and practical use of affinity chromatography
• Details common affinity methods such as bioaffinity, immunoaffinity, DNA, boronate, dye-ligand, biomimetic, and metal-ion affinity chromatography
• Discusses techniques used in clinical, environmental, and pharmaceutical analyses
• Illustrates the use of affinity chromatography for applications in biotechnology and molecular biology
• Explains quantitative approaches for the analysis of biological interactions
• Contains nearly 500 figures and tables while providing useful examples of affinity methods and their applications
• Offers over 3000 references to original papers available on topics of interest

The Handbook of Affinity Chromatography reflects upon important factors to consider in the development of affinity methods, such as the choice of support material, immobilization methods, and application or elution conditions. It reviews common affinity methods and explores the latest preparative, analytical, and biophysical applications, including the use of affinity chromatography with other separation techniques and analytical systems. This basis seamlessly supports the discussion of recent developments in techniques including the use of affinity ligands in capillary electrophoresis, mass spectrometry, microanalytical systems, and optical biosensors. New chapters feature expanded discussions on molecularly imprinted polymers and biomimetic ligands, chromatographic immunoassays, affinity-based immunoassays, affinity-based chiral stationary phases, and affinity ligands in multidimensional systems.

Engaging the collaboration of 48 scientists and students from 23 laboratories and organizations to present the latest information on affinity methods, the Handbook of Affinity Chromatography illustrates a wide range of applications and theory for scientists, students, and laboratory workers throughout the fields of chemistry and biology.

Contents

INTRODUCTION AND BASIC CONCEPTS
An Introduction to Affinity Chromatography; D.S. Hage and P.F. Ruhn
Support Materials for Affinity Chromatography; P. Gustavsson and P. Larsson
Immobilization Methods for Affinity Chromatography; H.S. Kim and D.S. Hage
Application and Elution in Affinity Chromatography; D.S. Hage, H. Xuan, and M.A. Nelson

GENERAL AFFINITY LIGANDS AND METHODS
Bioaffinity Chromatography; D.S. Hage, M. Bian, R. Burks, E. Karle, C. Ohnmacht, and C. Wa
Immunoaffinity Chromatography; D.S. Hage and T.M. Phillips
DNA Affinity Chromatography; R.A. Moxley, S. Oak, H. Gadgil, and H.W. Jarrett
Boronate Affinity Chromatography; X. Liu and W.H. Scouten
Dye-Ligand and Biomimetic Affinity Chromatography; N.E. Labrou, K. Mazitsos, and Y.D. Clonis
Immobilized Metal-Ion Affinity Chromatography; D. Todorova and M.A. Vijayalakshmi

PREPARATIVE APPLICATIONS
General Considerations in Preparative Affinity Chromatography; A. Subramanian
Affinity Chromatography of Enzymes; F. Friedberg and A.R. Rhoads
Isolation of Recombinant Proteins by Affinity Chromatography; A. Subramanian
Affinity Chromatography in Antibody and Antigen Purification; T.M. Phillips
Affinity Chromatography of Regulatory and Signal-Transducing Proteins; A.R. Rhoads and F. Friedberg
Receptor-Affinity Chromatography; P. Bailon, M. Nachman-Clewner, C. L. Spence, and G.K. Ehrlich

ANALYTICAL AND SEMIPREPARATIVE APPLICATIONS
Affinity Methods in Clinical and Pharmaceutical Analysis; Carrie A.C. Wolfe, W. Clarke and D.S. Hage
Affinity Chromatography in Biotechnology; N. Jordan and I. Krull
Environmental Analysis by Affinity Chromatography; M.A. Nelson and D.S. Hage
Affinity Chromatography in Molecular Biology; L.A. Jurado, S.Oak, Himanshu Gadgil, R.A. Moxley, and H.W. Jarrett
Affinity-Based Chiral Stationary Phases; S. Patel, I.W. Wainer and W.J. Lough

BIOPHYSICAL APPLICATIONS
Quantitative Affinity Chromatography: Practical Aspects; D.S. Hage and J. Chen
Quantitative Affinity Chromatography: Recent Theoretical Developments; D.J. Winzor
Chromatographic Studies of Molecular Recognition and Solute Binding to Enzymes and Plasma Proteins; S. Patel, I.W. Wainer and W.J. Lough
Affinity-Based Optical Biosensors; S.D. Long and D.G. Myszka

RECENT DEVELOPMENTS
Affinity Ligands in Capillary Electrophoresis; Niels H. H. Heegaard and C. Schou
Affinity Mass Spectrometry; C.J. Briscoe, W. Clarke and D.S. Hage
Microanalytical Methods Based on Affinity Chromatography; T.M. Phillips
Chromatographic Immunoassays; A.C. Moser and D.S. Hage
Molecularly Imprinted Polymers: Artificial Receptors for Affinity Separations; K. Haupt