Practical Enzymology

Softcover  XVI, 256 Pages  ISBN 3527304444      £45.00

A broad experimental introduction considering all essential aspects of enzymology, containing a theoretical introduction together with practical protocols. Essential reading for all scientists and practitioners working with enzymes.

From the contents

INTRODUCTION

ENZYME NOMENCLATURE

ENZYME REACTIONS
Theory of the enzyme reaction
Michaelis-Menten equation
Theory of the enzyme test
Theory of coupled enzyme reactions
Substrate determination
Instrumental aspects
Enzyme assays
Diverse methods
Enzyme immunoassays

BINDING MEASUREMENTS
Irreversible, reversible, specific and unspecific binding
Binding measurement by size discrimination
Spectroscopic techniques
Other methods for binding measurements

ENZYMES IN TECHNICAL APPLICATIONS
Principles of enzyme immobilisation
Methods for enzyme immobilisation
Analysis of immobilised enzymes
Enzyme reactors
Biosensors
Immobilized enzymes in therapy

APPENDIX
List of enzymes according to EC numbers