Biochemical Methods - A Concise Guide for Students and Researchers (with CD-ROM)

Hardcover with CD-ROM  XIV, 360 Pages  ISBN 3527302999      £65.00

The book gives a profound overview on the basics of relevant biochemical techniques. Moreover, it refers to laboratory equipment and safety aspects and explains how to obtain relevant biochemical information. It provides an introduction into physical-chemical processes and mathematical methods required for the interpretation of data. Principles of expensive instrumental analysis are explained.

With its practical approach the book is highly useful for professionals - laboratory technicians and scientists - but also for students.

Special feature: a CD-ROM on quantitative analysis of biochemical experiments!

"... An ideal how-to for those working in biochemistry."
CHEMIE in unserer Zeit

"... and anyone working in a biochemical laboratory will find it useful. Strongly recommended." Laboratory News

From the contents

PREFACE

BIOCHEMICAL LITERATURE
Accessing the biochemical literature
Access to the methodologically-based biochemical literature
Reference works and handbooks
Literature searches
Documentation of practical work
Literature

GENERAL LABORATORY PROCEDURES
The biochemistry laboratory
Routine biochemical procedures
Working with radioactivity
Literature

SAMPLE PREPARATION
Cell and tissue disruption
Solubilisation
Precipitation procedures for proteins and nucleic acids
Dialysis, ultrafiltration and lyophilisation
Literature

SEPARATION METHODS
Chromatography
Electrophoresis
Centrifugation
Literature

ANALYTICAL METHODS
Protein analysis
Nucleic acid analysis
Enzymatic analysis
Literature

IMMUNOLOGICAL METHODS
Antibodies
Antibody-antigen interactions
Literature

BIOPHYSICAL METHODS
Spectroscopy
Scattering techniques
Interactions
Determination of structure
Literature

MATHEMATICAL METHODS
Statistics
Quantitative evaluation of experimental results
Sequence analysis
Literature

QUANTITATIVE ANALYSIS OF BIOCHEMICAL DATA
Introduction
Applications
Guide to the compact disc

APPENDIX I: SI-UNITS

APPENDIX II: CONVERSIONS INTO SI-UNITS

INDEX

+ CD containing Chapter 9 and Exercises to Chapter 9