Mass Spectrometry of Biological Materials - Second Edition

Hard Cover  488 pages, Illustrated  ISBN 0824701577      £130.00

"...highly recommended, both as an essential text for newcomers to the field and as a valuable, integrated and up-to-date reference source for anybody interested in state-of-the-art research in mass spectrometry in biology, biochemistry and medicine."
International Journal of Mass Spectrometry and Ion Processes

"...warmly recommended - a stimulating reference text...It deserves to be on both laboratory and personal bookshelves!"
Trends in Analytical Chemistry

This timely Second Edition of an established resource covers the huge expansion of information that has taken place since the publication of the first edition€providing up-to-the-minute discussions on the application of mass spectrometry to the biological sciences.

Shows how and why experiments are performed and furnishes details to facilitate the duplication of results! Completely rewritten and enlarged by internationally recognized scientists from industry and academia, Mass Spectrometry of Biological Materials, Second Edition:

• reviews the ionization methods and instrumentation used in the analysis of biological materials
• introduces new instrumental technology that can solve fundamental problems in biology
• demonstrates techniques for isolating and identifying target molecules in complex mixtures that are often present in only trace amounts
• describes methods for characterizing proteins at very low levels of sample material
• analyzes noncovalent interactions and the use of hydrogen/deuterium exchange for tertiary structure determination
• explains the characterization of oligonucleotides and DNA
• supplies in-depth coverage of screening protein databases using data generated by mass spectrometry and more!

Containing over 800 bibliographic citations and illustrations, Mass Spectrometry of Biological Materials, Second Edition is a useful guide for mass spectrometrists; analytical, medicinal, and protein chemists and biochemists; biotechnologists; enzymologists; molecular biologists; agricultural scientists; and graduate-level and continuing-education students in these disciplines.

Contents

Instrumentation and Ionization Methods for the Analysis of Biological Materials by Mass Spectrometry, Charles N. McEwen and Barbara S. Larsen
High-Resolution Mass Spectrometry and Accurate Mass Measurements of Biopolymers Using MALDI-TOF, Ricky D. Edmondson and David H. Russell
A Novel Geometry Mass Spectrometer, the Quadrupole Orthogonal Acceleration Time-of-Flight Instrument, for Low Femtomole/Attomole Range Biopolymer Sequencing, Howard R. Morris, Thanai Paxton, Maria Panico, Roy McDowell, and Anne Dell
Affinity Techniques Combined with Mass Spectrometry, Ray Wieboldt, Jerry Zweigenbaum, and Jack D. Henion
Screening Molecular Diversity Using Pulsed Ultrafiltration Mass Spectrometry, Richard B. van Breemen, Charles P. Woodbury, and Duane L. Venton
Mass Spectrometry in Immunology: Identification of a Minor Histocompatibility Antigen, Carthene R. Bazemore Walker, Nicholas Sherman, Jeffrey Shabanowitz, and Donald F. Hunt
Strategies for the Rapid Characterization of Recombinant and Native Proteins by Mass Spectrometry, Daniel B. Kassel, R. Kevin Blackburn, and Bruno Antonsson
Mass Spectrometry in Drug Discovery, M. Arthur Moseley III, Douglas M. Sheeley, R. Kevin Blackburn, Robert L. Johnson, and Barbara M. Merrill
Analysis of Glycoproteins, Ron Orlando and Yi Yang
Analysis of Phosphorylated Proteins by Mass Spectrometry, Chhabil Dass
Mass Spectrometry-Based Approaches Toward the Study of Regulated Biological Systems, Julian D. Watts, Axel Ducret, Daniel Figeys, Ming Gu, Yanni Zhang, Paul A. Haynes, Rose Boyle, and Ruedi Aebersold
Gels in Vacuo? A Minimalist Approach for Combining Mass Spectrometry and Polyacrylamide Gel Electrophoresis, Rachel R. Ogorzalek Loo, Philip C. Andrews, and Joseph A. Loo
Studying Noncovalent Interactions by Electrospray Ionization Mass Spectrometry, Joseph A. Loo and Kristin A. Sannes-Lowery
Protein-Protein and Protein-Ligand Interactions Studied by Hydrogen/Deuterium Exchange Mass Spectrometry, Carol V. Robinson
Characterization of Oligonucleotides by Electrospray Mass Spectrometry, Pamela F. Crain
Methods and Applications of Sizing DNA by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry, David M. Lubman, Jian Bai, Yan-Hui Liu, Jannavi R. Srinivasan, Yongdong Zhu, David Siemieniak, and Patrick J. Venta
Network-Based Bioinformatics in Protein Mass Spectrometry, David Fenyö and Ronald C. Beavis