Protein Engineering for Industrial Biotechnology

388pp  ISBN 9057024128      £95.00

Protein engineering has proved to be one of the more fruitful technological approaches in biotechnology, being both very powerful and able to generate valuable intellectual property . The book aims to present examples in which the application of protein engineering has successfully solved problems arising in industrial biotechnology.

There is a section on its use to enhance purification of recombinant proteins. The use of protein engineering to modify the activity or the stability of industrial enzymes from lipases to proteases, from carboxypeptidases to glucanases and glucosidases, and from pectin modifying enzymes to enzymes able to degrade recalcitrant compounds is extensively covered. It is shown how areas as diverse as agrofood technology, fine chemistry, detergents, bioremediation and biosensors receive significant contributions from protein and solvent engineering.

The application of protein engineering to health care is also covered, from the development of new vaccines to new potential therapeutic proteins. A specific notation is given to protein engineering in the development of target molecules for drug discovery.

International in scope, the many contributions are drawn from academia and industry. The text should be of interest to students and researchers in industrial biotechnology as well as to everybody interested in basic research in protein structure, molecular genetics, bio-organic chemistry, biochemistry, agrobiotechnology, pharmaceutical sciences and medicine.

Contents

• Protein Engineering in Basic and Applied Biotechnology: A Review
• Enhanced Recovery and Folding of Recombinant Proteins Using Fusion Protein Strategies
• Protein Engineering for Affinity Purification: The Strep-tag
• Candida rugosa Lipases: From Molecular Evolution Analysis to the Design of a Synthetic Gene
• Structure-Function Studies on Cutinase, a Small Lipolytic Enzyme with a Water Accessible Active Site
• Lipases from rhizopus Species: Genetics, Structure and Applications
• Solvent Engineering Modulates Stereoselectivity of Microbial Lipases
• Stabilization of Industrial Enzymes by Protein Engineering
• Design of Biotechnologically Applicable Yeast Carboxypeptidase Y Mutants with Increased Preference for Charged P1 Residues
• Engineering ß-Glycoside Hydrolases
• Engineering Specificity and Stability in Glucoamylase from Aspergillus Niger
• Pectin Modifying Enzymes and Designer Pectins
• Engineering Proteins for the Degradation of Recalcitrant Compounds
• Protein Engineering for Biosensors
• Protein Engineering in Vaccine Development
• Exploring Molecular Recognition by Combinatorial and Rational Approaches
• Production and Potential Applications of Recombinant Gastric Lipasis in Biotechnology
• Inhibition of Ras-Dependent Signal Transduction for Therapeutic Purposes: Protein Engineering of a Ras-Specific Guanine Nucleotide Exchange Factor
• Expression and Use of Tagged Cells Surface Receptors for Drug Discovery
• Estrogen Receptor as a Target for New Drug Discovery
• Molecular Bioscreening in Oncology